Short chain fatty acids product of microbial fermentation modulate myeloid cell activation in vitro

ERREA A., IRAPORDA C., CAYET D.,; ABRAHAM A.G.,. ; GARROTE G.L., SIRARD J.C. RUMBO M

Congreso; . LIX Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC)-LXII Reunion anual de la Socidad Argentina de Inmunologia (SAI)..; 2014

Mucosal immune response may be modulated by microbial metabolites. The recent discovery of several signaling receptors able to sense short chain fatty acids (SCFA) have placed these molecules as putative mediators of host-micorbial interaction, instead of considering them as mere inert terminal metabolic products. SCFA are present in high levels in large intestinal lumen where they are produced by intestinal microbiota. The aim of this work was to determine if SCFA (butyrate, propionate, acetate and lactate) can modulate the properties of activated bone marrow derived macrophages (BMMs) or dendritic cells (BMDCs). C57BL/6 was used as bone marrow source. BMMs were derived upon 6 days differentiation in presence of M-CSF and BMDCs were derived upon 9 days of differentiation in presence of GM-CSF. Cells were stimulated with 1 ug/mL of E.coli LPS in presence of different concentrations of the different SCFA. Activation of BMDCs was monitored by changes in surface expression of CD40, CD80 and CD86 and IL6 and IL12 production upon overnight incubation. Secretion of IL6 and IL12 was evaluated as indicator of BMM stimulation.In all cases SCFA modulated surface expression of CD40 in BMDCs (p