Immunochemical Methods for Detection of Probiotics

ABRAHAM, ANALIA G; SERRADELL MARÍA; GARROTE GRACIELA; FOSSATI CARLOS A; DE ANTONI GRACIELA

Probiotic in Food Safety and Human Health

Taylor and Francis CRC Press

Lugar: United Kindom; Año: 2005; p. 165 - 189

 Contents Antigenic Molecules and Structures Associated to Probiotic Characteristics. Application of Immunochemical Techniques to Potentially Detect Probiotic Bacteria . Methodology for Immunochemical Detection of Probiotic. Preparation of Antigens. Preparation of Bacterial Antigens . Purification of Bacterial Proteins.  Production of Specific Antibodies. Production of Polyclonal Antibodies (Antisera). Preparation and Production of Monoclonal Antibodies. Labeling of Antibodies.  Labeling Antibodies with Fluorochromes. Labeling Antibodies with Biotin . Labeling Antibodies with Enzymes. Methods for Detection. Indirect Immunofluorescence. Noncompetitive Indirect ELISA. Competitive Sequential ELISA. Capture ELISA. Immunoblotting or Western Blot. Dot Blot. Flow Cytometry. Conclusions. References.