INVESTIGADORES
CHULUYAN Hector Eduardo
congresos y reuniones científicas
Título:
Slpi upregulate the expression of IL-6 and IL-10 in lymphocytes.
Autor/es:
GUERRIERI D, REITERI MACARENA, MAFFIA P, CHULUYAN E.
Lugar:
Cordoba, Argentina
Reunión:
Congreso; 7º congreso de la Asociacion Latinoamericana de Inmunologia; 2005
Institución organizadora:
ALAI
Resumen:
AbstractPrevious works in our laboratory have demonstrated
that Secretory Leucocyte Protease Inhibitor (SLPI) decreased the proliferation
of human T cells. In this study, we analyzed the expression of different
cytokines produced by lymphocytes treated with rhSLPI. In order to evaluate the capacity of SLPI to
modulate the pattern of expression of cytokines we use a BDTM CBA
Human Th1/Th2 Cytokine Kit II. This kit allows us to measure simultaneously
IL-2, IL-4, IL-6, IL-10, TNF-a and IFN-g by flow cytometry in the supernatant of
lymphocytes stimulated with rhSLPI (4 mg/ml, 5 days, 37ºC). The presence of rhSLPI increase
the expression of IL-6 (from 0,065±0,01 to 3,4±0,7 ng/ml) and IL-10 (from 0,02±0,01
to 0,4±0,1 ng/ml) meanwhile no
significant effect was observed on the others cytokines. We also observed that
IL-2 (8 ng/ml) reverted the increase produced by rhSLPI of IL-6 (1,4±0.7 ng/ml)
and IL-10 (0,3±0,04 ng/ml). Furthermore, rhSLPI (4 mg/ml) decreased the expression of T-box expressed in T-cell (T-bet) in lymphocytes stimulated with IL-2 (8
ng/ml) during 4 days through a Western blot. Since, T-bet expression is
implicated in generating Th1 type of immune response, overall these results suggest that SLPI can modulate the
expression of cytokines leading to Th2 cytokine pattern of expression.