Therapy with Dendritic Cells Influences the Spontaneous Resorption Rate in the CBA/J x DBA/2J Mouse Model.

BLOIS S, ALBA SOTO CD, OLMOS S, CHULUYAN HE, GENTILE T, ARCK PC, MARGNI RA.

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY

Año: 2004 vol. 51 p. 40 - 48

8755-8920

Abstract PROBLEM: DBA/2J-mate CBA/J female mice are prone to a high incidence of fetal abortions. This fetal wastage can be dramatically reduced by immunizing the female mice with BALB/c, but not with DBA/2J spleen cells during early gestation. Nevertheless, the underlying mechanisms remain to be elucidated. Recently, dendritic cells (DC) have been describe at the feto-maternal interface in the human uterus. In this work, we studied the effect of adoptive transfer of DC on the maintenance of pregnancy in the CBA/J · DBA/2J model. METHODS: Bone marrow-derived DC were generated from virgin female CBA/J mice (6–8 weeks ol ). CBA/J females were inoculated with DC twice before mating. Four different experimental groups were included : (i) no treatment control, (ii) mice injected with culture medium [granulocyte–macrophage colony-stimulating factor (GM-CSF)], (iii) immunized with DC an (iv) immunized with paternal DBA/2J antigens lisate-pulse DC, n = 5. RESULTS: The control abortion rate was 23.8%, an with GM-CSF alone was 17.6%. Following inoculation of syngeneic DC abortion rates were reduced to 2.2%, but protection was short-lived. Abortion rates with DC pulse with DBA/2J antigens was 5%. Serum of interleukin (IL)-6 levels were lower in the latter two groups up to the time of abortion. The kinetics of immunoglobulin G asymmetric antibodies synthesis was modified, but there was no correlation between asymmetric antibodies production an the lowering of abortions rates. CONCLUSION: Syngeneic DC prevented abortions an this was linked to a decrease in IL-6 levels, but not with levels of asymmetric antibodies.· DBA/2J model. METHODS: Bone marrow-derived DC were generated from virgin female CBA/J mice (6–8 weeks ol ). CBA/J females were inoculated with DC twice before mating. Four different experimental groups were included : (i) no treatment control, (ii) mice injected with culture medium [granulocyte–macrophage colony-stimulating factor (GM-CSF)], (iii) immunized with DC an (iv) immunized with paternal DBA/2J antigens lisate-pulse DC, n = 5. RESULTS: The control abortion rate was 23.8%, an with GM-CSF alone was 17.6%. Following inoculation of syngeneic DC abortion rates were reduced to 2.2%, but protection was short-lived. Abortion rates with DC pulse with DBA/2J antigens was 5%. Serum of interleukin (IL)-6 levels were lower in the latter two groups up to the time of abortion. The kinetics of immunoglobulin G asymmetric antibodies synthesis was modified, but there was no correlation between asymmetric antibodies production an the lowering of abortions rates. CONCLUSION: Syngeneic DC prevented abortions an this was linked to a decrease in IL-6 levels, but not with levels of asymmetric antibodies.