Characterization of Hg-phytochelatins complexes in vines (Vitis vinifera cv Malbec) as defense mechanism against metal stress

ADRIÁN A. SPISSO; SOLEDAD CERUTTI; FERNANDA SILVA; PABLO H. PACHECO; LUIS D. MARTÍNEZ

BIOMETALS

SPRINGER

Lugar: Berlin; Año: 2014

0966-0844

An approach to understand vines (Vitis vinifera) defense mechanism against heavy metalstress by isolation and determination of Hg-phytochelatins (PCs) complexes was performed. PCs are important molecules involved in the control of metal concentration in plants. PCs complex toxic metals through -SH groups and stores them inside cells vacuole avoiding any toxic effect of free metals in the cytosol. The Hg-PCs identification was achieved by determination of Hg and S as hetero-tagged atoms. A method involving two-dimensional chromatographic analysis coupled to atomic spectrometry and confirmation by tandem mass spectrometry is proposed. An approach involving size exclusion chromatography coupled to inductively coupled plasma mass spectrometryon roots, stems, and leaves extracts describing Hg distribution according to molecular weight andsulfur associations is proposed for the first time.