INVESTIGADORES
PACHECO Pablo Hugo
artículos
Título:
Trace aluminium determination in biological samples after microwave digestion followed by solid phase extraction with L-methionine on controlled pore glass
Autor/es:
PABLO H. PACHECO, RAÚL A. GIL, PATRICIA SMICHOWSKI, GRISELDA POLLA, LUIS D. MARTINEZ.
Revista:
MICROCHEMICAL JOURNAL
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2007 p. 1 - 6
ISSN:
0026-265X
Resumen:
Aluminium is an element found in biological samples at low concentrations and aluminum-related neuropathological diseases in human beings
have been reported. For this reason the determination of Al in this type of samples requires the development of enrichment methods capable of
improving the instrumental detection for this analyte.
L-methionine immobilized on controlled pore glass (CPG) was tested for the retention of aluminium. This adsorbent material was packed in a
conical minicolumn and connected to an on-line flow injection system. An inductively coupled plasma optical emission spectrometer associated
toan ultrasonic nebulization system (USN-ICP OES) was used for aluminium detection. Al(III) was retained at pH 12.5 and removed from the
column with 20% (v/v) HNO3.
The precision of the preconcentration method was evaluated by passing a measured volume of aluminium standard solution (1 ìg L-1) through
the minicolumn and repeating this procedure five times. The relative standard deviation (RSD) was 2.5%, calculated with the peak heights
obtained. A total enhancement factor of 1600 was attained for a sample volume of 10 mL (10-fold for the ultrasonic nebulization system and
160-fold for the preconcentration methodology). The detection limit (DL), calculated as the amount of Al required to yield a net peak equal to
three times the standard deviation of the blank solution, was 25 ng L-1.
The proposed system was successfully applied to the determination of aluminium in urine, hair, and saliva samples, which were pretreated
using a microwave digestion methodology with the introduction of a digestion program for saliva. The average aluminium levels found in
urine, hair, and saliva samples were 5.5 ìg L-1, 19 ìg g-1 and 93 ìg L-1 respectively.-methionine immobilized on controlled pore glass (CPG) was tested for the retention of aluminium. This adsorbent material was packed in a
conical minicolumn and connected to an on-line flow injection system. An inductively coupled plasma optical emission spectrometer associated
toan ultrasonic nebulization system (USN-ICP OES) was used for aluminium detection. Al(III) was retained at pH 12.5 and removed from the
column with 20% (v/v) HNO3.
The precision of the preconcentration method was evaluated by passing a measured volume of aluminium standard solution (1 ìg L-1) through
the minicolumn and repeating this procedure five times. The relative standard deviation (RSD) was 2.5%, calculated with the peak heights
obtained. A total enhancement factor of 1600 was attained for a sample volume of 10 mL (10-fold for the ultrasonic nebulization system and
160-fold for the preconcentration methodology). The detection limit (DL), calculated as the amount of Al required to yield a net peak equal to
three times the standard deviation of the blank solution, was 25 ng L-1.
The proposed system was successfully applied to the determination of aluminium in urine, hair, and saliva samples, which were pretreated
using a microwave digestion methodology with the introduction of a digestion program for saliva. The average aluminium levels found in
urine, hair, and saliva samples were 5.5 ìg L-1, 19 ìg g-1 and 93 ìg L-1 respectively.3.
The precision of the preconcentration method was evaluated by passing a measured volume of aluminium standard solution (1 ìg L-1) through
the minicolumn and repeating this procedure five times. The relative standard deviation (RSD) was 2.5%, calculated with the peak heights
obtained. A total enhancement factor of 1600 was attained for a sample volume of 10 mL (10-fold for the ultrasonic nebulization system and
160-fold for the preconcentration methodology). The detection limit (DL), calculated as the amount of Al required to yield a net peak equal to
three times the standard deviation of the blank solution, was 25 ng L-1.
The proposed system was successfully applied to the determination of aluminium in urine, hair, and saliva samples, which were pretreated
using a microwave digestion methodology with the introduction of a digestion program for saliva. The average aluminium levels found in
urine, hair, and saliva samples were 5.5 ìg L-1, 19 ìg g-1 and 93 ìg L-1 respectively.ìg L-1) through
the minicolumn and repeating this procedure five times. The relative standard deviation (RSD) was 2.5%, calculated with the peak heights
obtained. A total enhancement factor of 1600 was attained for a sample volume of 10 mL (10-fold for the ultrasonic nebulization system and
160-fold for the preconcentration methodology). The detection limit (DL), calculated as the amount of Al required to yield a net peak equal to
three times the standard deviation of the blank solution, was 25 ng L-1.
The proposed system was successfully applied to the determination of aluminium in urine, hair, and saliva samples, which were pretreated
using a microwave digestion methodology with the introduction of a digestion program for saliva. The average aluminium levels found in
urine, hair, and saliva samples were 5.5 ìg L-1, 19 ìg g-1 and 93 ìg L-1 respectively.-1.
The proposed system was successfully applied to the determination of aluminium in urine, hair, and saliva samples, which were pretreated
using a microwave digestion methodology with the introduction of a digestion program for saliva. The average aluminium levels found in
urine, hair, and saliva samples were 5.5 ìg L-1, 19 ìg g-1 and 93 ìg L-1 respectively.ìg L-1, 19 ìg g-1 and 93 ìg L-1 respectively.