Selective determination of inorganic selenium species in parenteral solutions using L-methionine as retaining agent in ETAAS

PABLO H. PACHECO, RAÚL A. GIL, PATRICIA SMICHOWSKI, GRISELDA POLLA, LUIS D. MARTINEZ.

JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY

ROYAL SOC CHEMISTRY

Año: 2008 p. 397 - 401

0267-9477

A time-based .ow injection (FI) separation system coupled to electrothermal atomic absorption spectroscopy (ETAAS) has been developed for the determination of selenium in parenteral solutions. Selenate interacts with L-methionine, which is immobilized on controlled pore glass (CPG), at pH 3.0 and eluted with HCl (10% v/v). Total selenium content is determined directly by ETAAS without preconcentration and selenite is calculated by di.erence. The features of the separation system are: 100% e.ciency of retention and an enhancement factor of 20 for a preconcentration time of 6 s (sample .ow rate = 10 mL min
1) with HCl elution volumes of 50 mL. The detection limit (3s) was 6 ng L
1, and the relative standard deviation (n = 10) at the 0.1 mg L
1 level is 3.0%. The method has high selectivity and was applied to the determination of Se (IV) and (VI) in parenteral solutions with satisfactory results.L-methionine, which is immobilized on controlled pore glass (CPG), at pH 3.0 and eluted with HCl (10% v/v). Total selenium content is determined directly by ETAAS without preconcentration and selenite is calculated by di.erence. The features of the separation system are: 100% e.ciency of retention and an enhancement factor of 20 for a preconcentration time of 6 s (sample .ow rate = 10 mL min
1) with HCl elution volumes of 50 mL. The detection limit (3s) was 6 ng L
1, and the relative standard deviation (n = 10) at the 0.1 mg L
1 level is 3.0%. The method has high selectivity and was applied to the determination of Se (IV) and (VI) in parenteral solutions with satisfactory results.
1) with HCl elution volumes of 50 mL. The detection limit (3s) was 6 ng L
1, and the relative standard deviation (n = 10) at the 0.1 mg L
1 level is 3.0%. The method has high selectivity and was applied to the determination of Se (IV) and (VI) in parenteral solutions with satisfactory results.s) was 6 ng L
1, and the relative standard deviation (n = 10) at the 0.1 mg L
1 level is 3.0%. The method has high selectivity and was applied to the determination of Se (IV) and (VI) in parenteral solutions with satisfactory results.mg L
1 level is 3.0%. The method has high selectivity and was applied to the determination of Se (IV) and (VI) in parenteral solutions with satisfactory results.IV) and (VI) in parenteral solutions with satisfactory results.