Anticonvulsant effects of propylparaben and sodium cyclamate in a model of PTZ-induced seizure in zebrafish

ALAN TALEVI; SOFÍA OTERO; RAMÓN BERNABEU; LUIS E. BRUNO-BLANCH

Cancún

Congreso; 9th Latin American Congress on Epilepsy; 2016

International League Against Epilepsy

Purpose:Currently, the primary screening for the identification of new anticonvulsant drugs which could be potentially applied in the therapy of epilepsy follows the lineaments of the National Institute of Neurological Disorders and Stroke, which in the first stage resorts to acute models of seizure in mice. The need of novel preclinical models has been indicated as one of the keys to develop innovative solutions for the treatment of epilepsy (Löscher W & Schmidt D. Epilepsia 2011). Danio rerio constitutes an accessiblemodel for high throughput screening. On the basis of previous results in mice models (Talevi A et al. J Comput Aided Mol Des 2007;21:527-538; Di Ianni ME et al. Comb Chem High Thr Screen 2015;18:335-345) we have studied, with comparative goals, the anticonvulsant effect propylparaben and sodium cyclamate in a model of PTZ-induced seizures in zebrafish.Method: Groups of 5-6 zebrafish were exposed to a 6 mM PTZ solution plus sodium cyclamate 1 μg/μL or propylparaben 9 μg/μL. Additionally, the effect of pre-exposure to the previously mentioned concentrations during 4 days was investigated, using valproic acids and saline as controls.Results: Sodium cyclamate significantly reduced PTZ-induced spasms (p