“Inhibición del crecimiento de Paenibacullus larvae subsp. larvae frente al aceite esencial de canela (Cinnamomun zeylanicum) y sus componentes

GENDE, LIESEL; FUSELLI, SANDRA; FRITZ, ROSALIA; EGUARAS, MARTIN

Punta del Este, Uruguay

Congreso; Primer Congreso de Apicultura del MERCOSUR; 2005

The antimicrobial activity of the essential oil of cinnamon (Cinnamomun Zeylanicum) against of Paenibacillus larvae subsp. larvae was studied. The oil was obtained by steam distillation. The components were analyzed by fine layer chromatography revealing rf 0.67 for the cinamic aldehyde, 0.35 for the eugenol and a rf area among 0.05 and 0.21 corresponding to the region of terpenic alcohols. The antimicrobial activity of the oil was determined by the tube dilution method in Mueller-Hinton broth for the Minimal inhibitory concentration (MIC) and agar MYPGP (broth Mueller-Hinton, yeast extract, glucose and piruvato of sodium) for the minimal bactericide concentration (MBC). On the other hand the section of inhibition taken place by the components of the essential oil using the bioautography method on a chromatogram included in agar MYPGP with trifenil tetrazolium chloride in water were determined. The chromatography badges developed covered with the means of inoculated cultivation were incubated at 36 (1ºC during 24-48 hours). The essential oil of cinnamon registered values between 50-100 mg/L and 100-125 mg/L of CIM and CBM respectively. In the bioautografhy method yellow areas of inhibition corresponding to the cromatography fraction with antimicrobial activity on the sections corresponding to the aldehyde cinámico and to the eugenol were observed. The essential oil of Cinamum zeylanicum (cinnamon) and especially two of their components presented inhibitory capacity in front of the strains of P. larvae subsp. larvae.