Prevalencia de Yersinia enterocolitica en carnes bovinas y porcinas

LUCERO ESTRADA CECILIA STELLA MARYS; VELÁZQUEZ LIDIA DEL CARMEN; STEFANINI DE GUZMÁN ANA MARÍA

Merlo, San Luís

Jornada; XXIII Reunión Científica Anual Sociedad de Biología de Cuyo; 2005

Sociedad de Biología de Cuyo

Yersinia enterocolitica is a food-born bacterium that can cause gastroenteritis in human. The propose of this work was investigated the prevalence of Y. enterocolitica in chicken and porcine products acquired in San Luis city. Twenty five g of sample were homogenized in 225 ml phosphate buffer saline (PBS) with addition of 1% sorbitol and 0.15% bile salt, incubated 21 days at 4°C. Characteristic colonies were analyzed by culture, biochemical and serological tests. The isolated strains were genotyping by ramdon amplification polymorphism polymerase chain reaction (RAPD-PCR), using the primer A08, and plasmid isolation was also made. From 117 chicken samples studied, 7 strains (6.9%) of Y. enterocolitica and 8 strains (6.8%) of Yersinia intermedia were isolated. From 144 pork samples studied 3 strains (2%) of Y. intermedia were isolated. The Y. enterocolitica strains belonged to biotype 1A, 2 of them showed a plasmid with a molecular weigh < 72-76 Kb. RAPD-PCR showed heterogenicity among the studied strains showing to be an acceptable subtyping method. Although the biotype 1A is considered nonpathogenic, epidemiologic and experimental data suggest that Y. enterocolitica biotype 1A is able to cause illness.