Whole 140S FMDV particles are needed to elicit specific cellular immunity in vivo and to stimulated recall responses in vitro

BUCAFUSCO, DANILO; DI GIACOMO, SEBASTIÁN; PEGA, JUAN FRANCO; STAFFORINI, GUILLERMINA; CAPOZZO, ALEJANDRA; PÉREZ FILGUEIRA, MARIANO

Jerez de la Frontera

Congreso; Appliance of science in the progressive control of FMD; 2012

The European Commission for the control of Foot-and-Mouth disease (EuFMD)

Introduction Cross-reactive IFN- responses against foot-and-mouth disease virus (FMDV) have been described by vaccinating cattle with one strain and studying recall responses against other strains in vitro, stimulating bovine whole-blood culture with FMDV antigens. Here we studied the relevance of viral integrity in the homo-and-heterotypic induction of cell-mediated immunity (CMI). Materials and methods In a first experiment, blood from 8 multi-vaccinated cattle was stimulated with sucrose-purified 140S particles (inactivated virus, O1Campos and A24 strains), or 12S subunits obtained by heat treatment. IFN- was quantified using a commercial ELISA. In a second experiment IFN- was quantified in plasma from in vitro-stimulated blood of O1 Campos (n=16) and A24 (n=10) monovalent-vaccinated animals. Results Stimulation of blood from multi-vaccinated cattle with 12S particles significantly decreased the ability of both viruses to induce the anamnestic production of IFN- (p