Foot-and-mouth disease vaccination induces cross-reactive IFN-gamma responses in cattle that are dependent on the integrity of the 140S particles

BUCAFUSCO, DANILO; DI GIACOMO, SEBASTIÁN; PEGA, JUAN FRANCO; SCHAMMAS, JUAN MANUEL; CARDOSO, NANCY; CAPOZZO, ALEJANDRA; PÉREZ FILGUEIRA, MARIANO

VIROLOGY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2015

0042-6822

Interferon- (IFN-) recall responses against foot-and-mouth disease virus (FMDV) in FMD vaccinated cattle are utilized to study T-lymphocyte immunity against this virus. In this report, a recall IFN- assay based on a commercial ELISA was set up using 308 samples obtained from naïve and vaccinated cattle. Further, the assay was used to study cross-reactive responses between different FMDV vaccine strains. Blood samples from cattle immunized with monovalent vaccines containing A24/Cruzeiro/Brazil/55 (A24/Cruzeiro), A/Argentina/2001 (A/Arg/01) or O1/Campos/Brazil/58 (O1/Campos) strains were tested using purified FMDV from homologous and heterologous strains. FMDV-induced IFN- production was fully dependent on the quantity and structural integrity of the stimulating antigen. A24/Cruzeiro strain was the most efficient IFN- inducer in all vaccinated animals for heterologous and homologous virus, both when included in the vaccine or as stimulating antigen. Accordingly, A24/Cruzeiro 140S particles were more stable than O1/Campos particles. These results show that IFN- production relies on the presence of whole FMDV particles that can maintain their integrity along the incubation process, for in vitro use; and throughout the vaccine´s shelf-life, when utilized in vivo.