MENZELLA Hugo Gabriel
congresos y reuniones científicas
?Optimizing enzymatic oil degumming efficiency: novel phosphatidylinositol-specific phospholipase c enzyme and degumming process development?
CERMINATI, SEBASTIAN; PAOLETTI, LUCIANA; PEIRU, SALVADOR; MENZELLA H.G; CASTELLI, MARÍA EUGENIA
Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017
In the last few years, there has been a constant increase inthe demand for oils to be used as a food and production offuels. This demand has generated a need for cost-effectivemethods for removing contaminating phospholipids, knownas gums, during the refining process. Traditionally, physicaland chemical degumming methods have been used. Morerecently, developments were made to use enzymatic degumming, which possesses several advantages over chemicaland physical methods such as minimal chemical waste andhigher yields of refined oil. Type C phosholipases (PLCs)provide a higher extra yield of oil compared to traditionalmethods, both by generating 1,2-diacylglicerol (DAG) fromphospholipids, which is miscible with triacylglycerols (TAGs),and by releasing the TAGs trapped as a consequence of thereduced volume of gums. Most of the PLCs reported in theliterature for oil degumming have specificity for phosphatidylcholine (PC) and/or phosphatidylethanolamine (PE), whichtogether represent ∼67% of the phospholipids present insoybean oil. Recently, we designed a novel PCPLC-Y, an engineered enzyme that can completely hydrolyze PC and PE.In this work, phosphatidylinositol-specific phospholipase C(PIPLC) candidates obtained from an in silico analysis wereevaluated for oil degumming expecting to formulate this newenzyme in combination with PCPLC-Y in order to increasethe efficiency of oil degumming. A PIPLC from Lysinibacillus sphaericus was shown to efficiently remove phosphatidylinositol, which represents ∼24% of the phospholipidsin crude oil, and when combined with PCPLC-Y, the threemajor phospholipids present in crude oil were completely hydrolyzed, providing an extra yield of oil greater than 2.1%,compared to standard methods. A remarkably efficient fedbatch Escherichia coli fermentation process producing ∼14g/L of the recombinant PIPLC enzyme was developed, whichmay facilitate the adoption of this cost-effective oil-refiningprocess.