MENZELLA Hugo Gabriel
congresos y reuniones científicas
PHOSPHOLIPASE C FOR SOYBEAN OIL DEGUMMING
CLAUDIA ELENA; PABLO RAVASI; SEBASTIAN CERMINATI; MARIA CASTELLI; SALVADOR PEIRU; HUGO MENZELLA
Congreso; SAIB 2014; 2014
Crude vegetable oils must be refined prior to use for human consumption or biodiesel production. Oil ?degumming? consists of phospholipids or ?gums? removal, and is the process that causes the major lost in oil refining industry. Enzymatic degumming emerged as a valuable alternative due to its increase in oil yield. In this process, phospholipases are used to hydrolyze phospholipids, generating products with increased water solubility making easier their separation from oil. The aim of our study is to produce a phospholipase C with high efficiency in industrial soybean oil degumming. We designed an expression strategy to obtain B. cereus phospholipase C and three individual mutants in Pichia pastoris. We determined kinetic parameters of the three mutant proteins in aqueous media and oil and compared them to the wt enzyme. We selected one enzyme that shows superior catalytic properties in oil. In addition, we optimized reaction parameters such as temperature, pH and enzyme dosage to completely remove major phospholipids from crude oil, setting up reaction conditions that can be scaled up to meet industrial needs.