INTECH   27907
INSTITUTO TECNOLOGICO DE CHASCOMUS
Unidad Ejecutora - UE
artículos
Título:
The potential of a DIVA-like recombinant vaccine composed by rNcSAG1 and rAtHsp81.2 against vertical transmission in a mouse model of congenital neosporosis.
Autor/es:
CORIGLIANO, MARIANA G.; LEGARRALDE, ARIEL; SANDER, VALERIA A.; BENGOA-LUONI, SOFIA A.; ALBARRAC├ŹN, ROMINA M.; CLEMENTE, MARINA; SANCHEZ-LOPEZ, EDWIN; GANUZA, AGUSTINA
Revista:
ACTA TROPICA
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2019 vol. 198
ISSN:
0001-706X
Resumen:
Neospora caninumis the etiological agent of neosporosis, a worldwide infectious disease recognized as the major cause of abortions and reproductive failures in livestock, responsible for significant economic losses in cattle industries. Currently, there are not cost-effective control options for this pathology, and the development of a vaccine involving new and integrated approaches is highly recommended. In this study, we evaluated the immunogenic and protective efficacy, as well as the potential DIVA (Differentiation of Infected from Vaccinated Animals) character of a recombinant subunit vaccine composed by the major surface antigen fromN. caninum (NcSAG1) and the carrier/adjuvant heat shock protein 81.2 fromArabidopsis thaliana(AtHsp81.2) in a mouse model of congenital neosporosis. BALB/c female mice were intraperitoneal (i.p.) immunized with a mixture of equimolar quantities of rNcSAG1 and rAtHSP81.2 or each protein alone (rNcSAG1 or rAtHsp81.2). The vaccine containing a mixture of rNcSAG1 and rAtHsp81.2 significantly enhanced the production of specific antirNcSAG1 total IgG (tIgG), IgG1 and IgG2a antibodies in immunized mice when compared to control groups (nonvaccinated and rAtHsp81.2 immunized mice) as well as to the group of mice immunized only with the antigen (rNcSAG1). In addition, partial protection against vertical transmission and improvement of the offspring survival time was observed in this group. On the other hand, rAtHsp81.2 induced the production of specific antirAtHsp81.2 tIgG, allowing us to differentiate vaccinated from infected mice. Despite further experiments have tobe made in cattle to test the capability of this vaccine formulation to differentiate vaccinated from infected animals in thefield, our results suggest that the formulation composed by rNcSAG1 and rAtHsp81.2 could serve as a basis for the development of a new vaccine approach against bovine neosporosis.