. SSR markers for analysing South American Nothofagus species

BODENES C; CARON H; AZPILICUETA M M; GALLO L A

SILVAE GENETICA

J D SAUERLANDERS VERLAG

Año: 2021

0037-5349

11 newly discovered microsatellites were used to identifySSR markers for characterising South American Nothofagusspecies. This was carried out in six species. The sample sizesused were between four and six individuals per species. Thecross-genera transferability of 34 Quercus SSRs was alsoessayed. Out of the 11 new microsatellite markers, threeproved to be polymorphic (NnBIO 11, NgBIO 13 and NgBIO14). The qualitative confirmation of the inheritance of thesemarkers could also be verified. Polymorphism was alsoobserved in five of the cross-genera transferred SSRs (QrBIO7,quru-GA-0A01, quru-GA-0C11, quru-GA-0I01, quru-GA-0M07).The number of alleles per locus found range between 1 and 6per species. The eight polymorphic SSRs identified in thisstudy will constitute a valuable tool in the gene flow studiesthat are currently being carried out in natural populations ofSouth American Nothofagus species. The confirmation of crossspeciesand cross-genera transferability opens the way for theuse of SSRs as bridge markers in genetic mapping.