IFAB   27864
INSTITUTO DE INVESTIGACIONES FORESTALES Y AGROPECUARIAS BARILOCHE
Unidad Ejecutora - UE
artículos
Título:
LUTEOTROPHIC EFFECT OF GNRH OR HCG ON CORPUS LUTEUM FUNCTIONALITY DURING THE LUTEAL PHASE IN EWES
Autor/es:
FERNANDEZ J; LUZBEL DE LA SOTA; NATALIA SALVETTI; ISABEL LACAU- MENGIDO; MACARENA BRUNO GALARRAGA; MARCELA CUETO; HUGO ORTEGA; ANDRES SOTO; ULISES NOTARO; ALEJANDRO GIBBONS
Revista:
MEDICINA
Editorial:
Medicina
Referencias:
Lugar: Buenos Aires; Año: 2020
ISSN:
0025-7680
Resumen:
Different therapeutic strategies have been used with the objective of increasing the concentration of progesterone (P4) and improving luteal function in order to reduce embryonic losses. The objective of the study was to determine the effect of gonadotrophin releasing hormone (GnRH) or human chorionic gonadotrophin (hCG) treatment at 4 days after timed artificial insemination (TAI) on the induction of accessory corpora lutea (acc-CL), the production and synthesis of P4. A total of 27 adult Merino ewes were randomly assigned to three groups on day 4 post TAI: GnRH group (n= 9; 4 μg IM of GnRH analogue, Receptal®, Intervet, Argentine), hCG group (n= 9; 300 IU IM of hCG, Gonacor®, Ferring, Argentine) and Control group (n= 9; 1 ml IM of saline solution). Laparoscopic observations of the ovaries on days 4 and 10 post TAI were performed to determine the presence of ovulatory CL (o-CL) and acc-CL, respectively. Serum P4 concentration was assessed by chemiluminescence on days 4, 7 and 14 post TAI. On day 14 post TAI, o-CL and acc-CL were removed (n= 5 ewes per each treatment) to determine STAR and 3ß-hydroxysteroid dehydrogenase (3ß-HSD) expressions by immunohistochemistry. The results were analyzed by ANOVA. The GnRH and hCG treated groups had a higher number of acc-CL compared to the control group (1.3 ± 0.1 vs. 0.0 ± 0.0, p