Development of Poly-NIsopropylacrylamide Surfaces for the Selection of Swine Sperm

MORILLA GRICELDA; CAPELLA, VIRGINIA; BOSCH, PABLO; BLOIS DAMIÁN; BARBERO, CÉSAR A.; LIAUDAT, ANA CECILIA; RIVAROLA, CLAUDIA R.; RODRIGUEZ, NANCY

Corpus Journal of Dairy and Veterinary Science (CJDVS)

Corpus Publishers

Lugar: Melbourne; Año: 2021 vol. 2 p. 1 - 5

Th reproductive effiency of pig farms is directly correlated with the fertility of the boars. Th aim ofthis work was to develop polymeric materials that can be used as a platform to select a subpopulation of spermwith better cell physiological parameters. Polymeric hydrogels composed of Poly-N-isopropylacrylamidewith diffrent positive charges given by copolymerization with (3-acrylamidopropyl) trimethylammoniumchloride (APTA, 5-10-15%), were synthesized. Subsequently, the interaction between the sperm cells and thepolymeric surfaces was analyzed in TALP medium. Release of the spermatozoa from the polymeric surfaceswas induced by changing to Ca2+ free media. Sperm motility, cell viability, plasma membrane and acrosomeintegrity were evaluated. Th results indicated that a higher percentage of swine sperm attached to PNIPAMco-15% APTA hydrogels (62.86±3.33%). Ninety seven percent (97.19±1.45 %) of the sperm released from thePNIPAM co-15%APTA surfaces were viable (p