50. Development of Poly-N-Isopropylacrylamide Surfaces for the Selection of Swine Sperm.

LIAUDAT CECILIA; RIVAROLA CLAUDIA R.; RODRIGUEZ, NANCY; MORILLA, GRICELDA; CAPELLA, VIRGINIA; BOSCH, PABLO; BLOIS, DAMIAN; BARBERO, CESAR

Corpus Journal of Dairy and Veterinary Science (CJDVS)

American Dairy Science Association

Año: 2021

2576-9162

The reproductive efficiency of pig farms is directly correlated with the fertility of the boars. The aim of this work was to develop polymeric materials that can be used as a platform to select a subpopulation of sperm with better cell physiological parameters. Polymeric hydrogels composed of Poly-N-isopropylacrylamide with different positive charges given by copolymerization with (3-acrylamidopropyl) trimethylammonium chloride (APTA, 5-10-15%), were synthesized. Subsequently, the interaction between the sperm cells and the polymeric surfaces was analyzed in TALP medium. Release of the spermatozoa from the polymeric surfaces was induced by changing to Ca2+ free media. Sperm motility, cell viability, plasma membrane and acrosome integrity were evaluated. The results indicated that a higher percentage of swine sperm attached to PNIPAM-co-15% APTA hydrogels (62.86±3.33%). Ninety seven percent (97.19±1.45 %) of the sperm released from thePNIPAM co-15%APTA surfaces were viable (p