Dam Early Free Access to Hypertonic NaCl Solution Induces a Long- Term Effect on Offspring Basal Chronic Brain Cell Activity

MACAGNO, A; CAEIRO, XE; PORCARI, CY; VIVAS, L.; GODINO, A; MACCHIONE, AF

La Habana

Congreso; 2ND PAN-AMERICAN CONGRESS OF PHYSIOLOGICAL SCIENCES ?Physiology without borders?; 2019

Exposure to an altered osmotic environment during a pre/postnatal period candifferentially program the fluid intake and excretion pattern profile in a way thatpersists until adulthood. Our recent results indicate that maternal voluntary ingestionof hypertonic NaCl solution during pregnancy and lactation until one week postweaningalters the offspring?s central osmoregulatory mechanisms. Offspring waterand sodium intake induced by different osmotic manipulations (sodium depletion orsodium overload) is changed during adulthood (Macchione et al., 2012; 2015).However, the analysis of the programing effects on basal pattern of neuronal activityalong brain nuclei involved in the control of hydroelectrolyte balance is limited. Ouraim was to evaluate the impact of maternal voluntary ingestion of hypertonic NaClsolution (as previously detailed) in the basal brain chronic neuronal activity of adult?soffspring. Animal handling and experimental procedures were approved by the AnimalCare and Use Committee of our institute, and the National Institutes of Health (NIH)Guidelines were followed. We analyzed the immunohistochemical detection of brainFra like protein (Fra-LI), alone or combined with vasopressin (AVP) in control (M-Ctrolgroup) and imprinted (M-Na group) rats, at 70 postnatal day.Our results indicate that, the M-Na group showed increased Fra-Li immunoreactivity(ir) along the organum vasculosum of the lamina terminalis (OLVT) (M-Ctrol= 105.75±21.77 vs M-Na= 308.5 ±59.01; p=0.018; t=-3.22). However, double Fra-AVP ir neuronsor Fra-Li ir along the subfornical organ, hypothalamic paraventricular nucleus (PVN,lateral mangocellular and medial parvocellular subnuclei) and supraoptic nucleus(anterior and medial sections) did not show any significant differences between thematernal treatments.Taking into account our previous evidence and the present results indicate that theavailability of a rich source of sodium during the pre/postnatal period induces a longtermeffect on drinking and the basal neural activity along the OVLT implicated in thecontrol of hydroelectrolyte balance.