Phased gene expression profiles of the cell cycle in human leukemic cell lines.

RUSTICI G; DE SIERVI A; GARDNER K

USA

Congreso; 96th AACR Annual Meeting: American Association for Cancer Research.; 2006

Changes in programs of cellular behavior occur through a complicated orchestration of the expression and function of thousands of genes and their protein products. A full understanding of this process cannot be achieved through traditional "one gene in one experiment" approaches. Current DNA microarray technology provides a powerful platform for monitoring the changing expression patterns of thousands of genes, thus enabling researchers to view a global picture of the molecular events governing complex cellular processes. In this study we apply the Affymetrix whole human genome expression arrays (U133 plus 2.0) to analyze the transcriptional profiles of the entire homo sapiens gene set during the cell cycle in the leukemia cell lines Jurkat and K562, following synchronization by centrifugal elutriation. The use of elutriated cells provides a means of assessing changes in transcriptional profiles in the absence of the confounding influences of mitotic poisons or serum deprivation. We are integrating the multivariate analysis of this data with several complementary methods including phylogenetic conservation and proteomic profiling to define both transcriptional factors and gene regulatory sequences that play critical roles in the spatial and kinetic changes in gene expression during the cell cycle. This project will provide a valuable dataset of time-series annotated gene expression that will aid in identifying new functional genes clusters underlying an essential cellular process common to all eukaryotic cells.