Kinetic profiles of p300 occupancy in vivo predict common features of promoter structure and coactivator recruitment.

SMITH J; FEEBERN WJ; COLLINS I; DE SIERVI A; MONTANO I; HAGGERTY CM; MCNUTT MC; BUTSCHER WG; DZEKUNOVA I; PETERSEN DW; KAWASAKI E; MERCHANT JL; GARDNER K

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Año: 2004 vol. 101 p. 11554 - 11559

0027-8424

Understanding the language encrypted in the gene regulatory regions of the human genome is a challenging goal for the genomic era. Although customary extrapolations from steady-state mRNA levels have been effective, deciphering these regulatory codes will require additional empirical data sets that more closely reflect the dynamic progression of molecular events responsible for inducible transcription. We describe an approach using chromatin immunoprecipitation to profile the kinetic occupancy of the transcriptional coactivator and histone acetyltransferase p300 at numerous mitogen-induced genes in activated T cells. Comparison of these profiles reveals a class of promoters that share common patterns of inducible expression, p300 recruitment, dependence on selective p300 domains, and sensitivity to histone deacetylase inhibitors. Remarkably, this class also shares an evolutionarily conserved promoter composition and structure that accurately predicts additional human genes with similar functional attributes. This "reverse genomic" approach will have broad application for the genome-wide classification of promoter structure and function.