Oral immunotherapy using soy proteins modulates the allergic response in a mouse model of cow´s milk allergy

SMALDINI, PAOLA L; ORSINI DELGADO, LUCIA; CURCIARELLO RENATA; CANDREVA ANGELA MARÍA; FOSSATI ALBERTO; DOCENA GUILLERMO H.

Lima

Congreso; X° Congreso Latinoamericano de Inmunología - INMUNO PERU 2012; 2012

Asociación Latinoamericana de Inmunología

Food allergy is caused by a breakdown in immunologic tolerance mechanisms. There is no approved therapy for food allergies. In this work we aimed to induce oral tolerance to cow`s milk proteins (CMP) using CMP and cross-reactive soy proteins (SP) in a food allergy mouse model. Balb/c mice were gavaged for a week with CMP (CMP Tol) or with SP (PS Tol) prior to sensitization by oral administration of CMP with choleric toxin. Finally, mice were orally challenged with CMP. Immune response was evaluated with in vitro (serum specific IgE, IgG1, IgG2a and IgA level; cytokine secretion and mRNA expression, and induction of Foxp3 T cells in the intestinal mucosa and splenocytes) and in vivo assays (clinical score and cutaneous tests). We found a lower medium clinical score in tolerized mice with CMP or PS after the oral challenge than in sensitized mice, with a negative cutaneous test in tolerized mice. Specific IgE [OD 1,57±0,2 vs 1,06±0.3 (SP Tol) or 0,52±0,1 (CMP Tol); p