INVESTIGADORES
CURCIARELLO Renata
artículos
Título:
Cross-reactivity between the soybean protein p34 and bovine caseins.
Autor/es:
CANDREVA ANGELA MARÍA; SMALDINI PAOLA L; CURCIARELLO RENATA; CAHUERFF ANA; FOSSATI C. ALBERTO; DOCENA GUILLERMO H.; PETRUCCELLI SILVANA
Revista:
Allergy, Asthma & Immunology Research
Editorial:
Korean Academy of Asthma, Allergy and Clinical Immunology; Korean Academy of Pediatric Allergy and Respiratory Disease
Referencias:
Año: 2014 vol. 7 p. 60 - 68
Resumen:
Abstract
PURPOSE:
Soy-based
formulas are widely used as dairy substitutes to treat milk allergy
patients. However, reactions to soy have been reported in a small
proportion of patients with IgE-mediated milk allergies. The aim of this
work was to explore whether P34, a mayor soybean allergen, is involved
in this cross-reactivity.
METHODS:
In
vitro recognition of P34 was evaluated by immunoblotting, competitive
ELISA and basophil activation tests (BAT) using sera from allergic
patients. In vivo cross-reactivity was examined using an IgE-mediated
milk allergy mouse model.
RESULTS:
P34
was recognized by IgE antibodies from the sera of milk allergic
patients, casein-specific monoclonal antibodies, and sera from
milk-allergic mice. Spleen cells from sensitized mice incubated with
milk, soy or P34 secreted IL-5 and IL-13, while IFN-γ remained
unchanged. In addition, the cutaneous test was positive with cow's milk
proteins (CMP) and P34 in the milk allergy mouse model. Moreover,
milk-sensitized mice developed immediate symptoms following sublingual
exposure to P34.
CONCLUSIONS:
Our
results demonstrate that P34 shares epitopes with bovine casein, which
is responsible for inducing hypersensitivity symptoms in milk allergic
mice. This is the first report of the in vivo cross-allergenicity of
P34.