DIVERSITY OF COLD-ADAPTED FUNGI IN NON-POLAR GLACIERS

DE GARCÍA, VIRGINIA; LIBKIND, DIEGO; DUO SAITO, RUBÍ AZUL

San Carlos de Bariloche

Simposio; 34TH INTERNATIONAL SPECIALIZED SYMPOSIUM ON YEASTS; 2018

IPATEC, PROBIEN

Cold environments are one of the most widespread extreme environments, differentmicroorganisms including yeasts are able to survive, live and colonize theseenvironments. The objective of this work was to analyze the biodiversity of cold-tolerant cultivable yeasts associated with glaciers of Mount Tronador and compare them with free culture analysis of the same environment. Soil, ice and water samples were collected from Castaño Overa and Ventisquero Negro glaciers (Mount Tronador). Ice and water samples were filtered in 45um filters, and placed in petri dishes with 10% MYP medium and YPD with antibiotics. Soil samples were cultivated by means of serial dilutions in YPD and MYP media, with Rose Bengal and antibiotics. All samples were incubated at 5 ° C until the developments of colonies. For total DNA extractions, MOBIO® kits were used. Identified isolates yeasts corresponded to the genera:Solicoccozyma, Filobasidium, Naganishia, and Vishniacozyma among the mostabundant Basidiomycetes, and Candida and Aureobasidium for Ascomycetous. Freeculture method show a high abundance of Microbotryomycetes, and sequences ofTremellomycetes as Naganishia friedmannii and Filobasidium magnum were alsoabundant. Groups belonging to Chytridiomycetes were also registered. Identifiedspecies correspond to cosmopolitan, psychrophilic or psychrotolerant taxa. This study contributes to a better knowledge of the biodiversity of extremophile yeasts and to the preservation of genetic resources that allow the development of conservation strategies.