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Experimental evolution has been successfully applied for increase production of relevant secondary metabolites in microorganisms. Phaffia rhodozyma is a red basidiomycetous yeast capable of synthesizing two compounds with high value in marine and cosmetic industry: carotenoid pigments and mycosporine-glutaminol-glucoside (MGG). Both metabolites exert protection against UV radiation and have in vitro and in vivo antioxidant activity against reactive oxygen species. The aim of this work is to obtain MGG and carotenoids hyper-producers mutants from P. rhodozyma by means of experimental evolution, using reactive oxygen species and UV exposure as selective pressure.Three populations of P. rhodozyma strains CRUB 1149 and MUT 7918, were grown in 24-well microplates for 120 hs (11 duplications approximately). Cells were expose to subhinhibitory concentrations of H2O2 and UV radiation. In every serial transfer to a new well, each stress was increase by 10%. Periodically, samples were taken for cryopreservation, and determination of carotenoid pigments and MGG production and compared with the parental strains. After 165 generations exposure to UV radiation, we could obtain a mutant derived from MUT 7918 that accumulates 90 mg/g dry weight of MGG, corresponding to a 3-fold increase in MGG accumulation with respect to its parental strain (29 mg/g dry weight). Populations of MUT 7918 exposed to hydrogen peroxide showed no differences in MGG accumulation with respect to the parental strain. On the contrary, from CRUB 1149, only a population evolved in the presence of hydrogen peroxide register enhanced MGG accumulation (1,5-fold increase). So far, we have been able to apply an experimental evolution strategy for increase production of MGG in P. rhodozyma, obtaining two hyper-producers mutants. Further studies of carotenoid pigment production and stability of strains are being carried out.