Identification and characterization of differential gene expression in white blood cells exposed to ionizing radiation

VANESA BIOLATTI; IRENE LAURA IBAÑEZ; NICOLAS BELLORA

Buenos Aires

Congreso; Joint Meeting of Bioscience Societies 2017; 2017

Argentine Society of Clinical Investigation (SAIC)

The analysis of gene expression in blood cells has been emerged as a novel method for biodosimetry marker detection. The differential expressed genes could predict the clinical outcome of patients and guide the prevention, early diagnosis and treatment of ionizing radiation induced damage, improving traditional approaches. The aim of this study was to perform a meta-analysis of expression microarray data of irradiated blood cells in order to identify differentially expressed genes with overlapping among studies, which could be proposed as biomarkers of ionizing radiation exposure at 2 Gy and 24 h post-exposure. Data of four expression microarrays obtained from Gene Expression Omnibus (GEO) were evaluated by using the R programming language and Bioconductor packages. Differential gene expression was evaluated by Limma (parameters: lfc=1 and p