Gene expression involved in the cytoprotective and inflammatory response in acute leukemia

ISSÉ B; MÓNACO ME; LAZARTE SS; TERÁN MM; AGUERO AGUILERA AC; LEDESMA ACHEM ME; HARO C

Buenos Aires

Otro; LXV REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA; 2020

Sociedad Argentina de Investigaciones Clinicas

The generation of reactive oxygen species and cytoprotective response play an important role in perpetuity of acute leukemia (AL). The aim of this work was to study the behavior of antioxidant enzymes and pro-inflammatory cytokines at transcriptional level, and relate it with the transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) expression in AL. A descriptive study was conducted between September 2016 - July 2019. Ninety-three subjects (54 with AL and 39 apparently healthy individuals) were evaluated at the Universidad Nacional de Tucumán. The leukemia characterization was performed by: blood count, cytochemistry and flow cytometry. Malondialdehyde (MDA) levels and catalase (CAT) serum activity were determined. Transcription factor forkhead homeobox type O (FoxO3a) and Nrf2, antioxidant enzymes CAT, peroxiredoxin-2 (PRX-2) and superoxide dismutase (SOD), and the cytokines tumor necrosis factor-alpha (TNF-α) and interleukine-6 (IL-6) gene expression were analyzed with real-time PCR in peripheral mononuclear blood cells. Statistical analyses were performed by SPSS V.25 statistical software. The comparative study was performed using the Kolmogorov-Smirnov non-parametric test. All statistical analyses were considered significant at p