NRF2 TRANSCRIPTION FACTOR: ROLE AS A REGULATOR OF THE OXIDATIVE STATE IN BETA TALASEMIA MINOR

EMILSE, LEDESMA ACHEM MIRYAM; LAZARTE S; CECILIA, HARO; ALVAREZ ASENSIO , N; ME. MONACO; MAGDALENA M TERAN; AGÜERO AGUILERA AC; ISSÉ BLANCA ALICIA

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Jornada; XXXVI Jornadas de biología de la asociacion de biología de Tucumán; 2019

Asociación de Biología de Tucumán

The transcription factor Nrf-2 plays a crucial role as one of the cellular defense systems, which activates its response to inflammation and oxidative stress.Objectives: to study the role of Nrf-2 as a modulator of the expression of genes involved in cytoprotection in patients with beta thalassemic trait (RBT).Methodology: 26 individuals with RBT and 41 controls analyzed at the Instituto de bioquímica aplicada of the UNT, during the period 2016-2018. Molecular characterization of RBT involved the detection of mutations: Cd39, IVSI-1, IVISI-110 and IVISI-6. Thiobarbituric acid reactive species (TBARS), superoxide dismutase activity (SOD), interleukin-6 concentration (IL6) were determined and gene expression of the transcription factors Forkhead Box O3 (FoxO3) and Nuclear Factor Erythroid 2- related factor (Nrf-2), cytoprotective enzymes, catalase (CAT), superoxide dismutase (SOD), peroxiredoxin-2 (Prx-2) and proinflammatory cytokines: IL-6 and tumor necrosis factor alpha (TNF-α) by time PCR real.Results: the most frequent mutation was IVSI-110 in 30.8% (8/26) of the cases. At a systemic level, SOD activity and TBARS levels were significantly higher in RBT patients compared to the control group (p