Effect of lipid peroxidation on spermatal parameters in men in reproductive age

BONILLA F; HARO C; ALVAREZ ASENCIO N

Tafi de Valle

Jornada; XXXV Jornadas Científicas de la Asociación de Biología de Tucumán; 2018

Asociacion de Biologia de Tucuman

Reactive oxygen species (EROS) play an important role in a variety of cellular processes such as sperm maturation, chemotaxis, zona pellucida junction, acrosome reaction among others. An excessive production of EROS and / or a deficiency in the antioxidant defense generate a state known as oxidative stress that damages proteins, lipids and nucleic acids. At the level of the spermatozoon, the EROS produce lipid peroxidation reducing the fluidity of the plasma membrane and affecting its motility, a condition that would affect its fertilizing capacity.Objective: To study the influence of lipid peroxidation on motility and sperm morphology in men of reproductive age. Methods: 25 semen samples of men aged between 25 and 45 years were studied, which were divided into two groups: Men with Reproductive Disorder (HTR) and apparently healthy controls (C). The seminal parameters were evaluated according to the criteria established by the WHO 2010. In seminal plasma reactive species of thiobarbituric acid (TBARS) were determined using the technique of Beuge and Aust.Results: The HTR group showed significantly higher levels of TBARS compared to group C (TBARS µmol/L HTR = 2.6 ± 0.6, C = 1.8 ± 0.2), however the progressive motility and the sperm count were similar in both groups. The strict Kruger morphology was significantly lower in the HTR group compared to the control individuals (p