Balance redox en beta talasemia menor

HARO C; LAZARTE S; LEDESMA ACHEM ME; TERÁN MM; ISSE B; ALVAREZ ASENCIO N; MÓNACO ME

Tafi de Valle

Jornada; XXXV Jornadas Científicas de la Asociación de Biología de Tucumán; 2018

Asociacion de Biologia de Tucuman

Excess of reactive oxygen species generates an oxidative imbalance that leads to a shorter survival of the red blood cells aggravating the anemic state in patients with beta thalassemic trait (RBT).Objectives: To evaluate the redox balance at a systemic and transcriptional level in RBT patients.Methodology: Sixteen individuals with β-thalassemia minor and 12 apparently healthy was analyze in the Universidad Nacional at Tucumán between of 2016-2017. Reactive species of thiobarbituric acid (TBARS) and superoxide dismutase (SOD) were determined at the systemic level. The expression of the transcription factors Forkhead BoxO3 (FoxO3) and Nuclear Factor Erythroid 2- related factor (Nrf-2) and SOD by RetroTranscription-PCR was evaluated at gene level.Results: The systemic level, significantly higher MDA and SOD levels were found in the RBT patients compared to the control group, which indicates an increase in lipid peroxidation and high SOD activity as a consequence of their purifying response to the oxidation-reduction imbalance. At the gene level, the RBT group showed significantly higher levels of expression for Nrf-2 and SOD compared to the control group (p 0.05).Conclusion: The results obtained suggest an increase in oxidative stress which would act as an important factor in the behavior and severity of anemia in subjects with β-thalassemia. Our results shed new light on the mechanisms of adaptation against oxidative stress mediated through the Nrf-2 pathway which together with SOD could reduce the oxidative damage present in this pathology.