Estudio de la expresión del factor de transcripción FoxO3 y de peroxiredoxina-2 en leucemias agudas

AGUERO AGUILERA AC; TERAN M; ISSÉ B; LAYÚS B; LEDESMA ACHEM E; HARO C; MÓNACO ME; LAZARTE S

San Miguel de Tucuman

Jornada; XXXIV Jornadas Científicas de la Asociación de Biología de Tucumán; 2017

Asociacion de Biologia de Tucuman

Acute leukemias (LA) are accompanied by excessive production of reactive oxygen species (ROS) and / or a deficiency in antioxidant defenses, leading to a state known as oxidative stress (EOx). The forkhead homeobox type O (FOXO) transcription factors are a superfamily of proteins that regulate the expression of genes important for diverse cellular processes, being FOXO3 key in the resistance to the EOx since it would stimulate the transcription of purifying enzymes of EROs. Objective: Study the expression of the FOXO3 gene and its target enzyme Peroxiredoxin-2 (PRX-2) in LA patients. Methods: Between June 2015 and 2016, 14 patients with LA and 14 controls were studied at the Institute of Applied Biochemistry of UNT. The characterization of the leukemias was performed by: blood count, cytochemistry and flow cytometry. Gene expression was analyzed by RetroTranscription-PCR real-time using glyceraldehyde-3-phosphate dehydrogenase as endogenous control. Results: Of the total LA studied, 43% were of myeloid origin and 57% were lymphoid. The expression of FOXO3 was significantly lower in the LA group than in the control subjects (p