FUNGAL BIOPESTICIDES OBTAINED FROM CULTURES OF Fusarium sp. 3300 (ENTOMOPATHOGENIC FUNGUS)

MURUA G; ARENA MARIO EDUARDO; MARCINKEVICIUS K; GASTAMINZA G; VERA N; ANALÍA SALVATORE; ELENA CARTAGENA

Congreso; XXXIII ANNUAL SCIENTIFIC MEETING; 2017

Entomopathogenic fungi (HE) are the main biological agents used in integrated pest management (IPM). More than 13 species of Fusarium were described as insect pathogens. The aim of this study was to evaluate the insecticidal activity of extracts from culture Fusarium sp. 3300, on Spodoptera frugiperda (Lepidoptera), Ceratitis capitata (Diptera) and Tribolium castaneum (Coleoptera), insect pests that generate serious economic losses in northwestern Argentina. Fungal development was performed in a 1% glucosed potato cultivation medium (CPG) inoculated with 3% of spores (w / v) (107 spores / mL) of HE and with and without the addition of 1% (w / p) insect remains. The extracts were obtained by extracting the biomass with ethyl acetate (AcOEt) and methanol at a 1:1 ratio and the supernatant with AcOEt. Supernatant‟s ethyl acetate extract of Fungus-insect (FSHIAc) produced the greatest intake inhibition in conditions of choice on S. frugiperda (IIA 83%). High percentages of pupal mortality (35-50%) were observed. All tested extracts were moderate to strong inhibitors of oviposition of Ceratitis capitata, the most active extract FSHIAc (IIO 61%). The Fungus-Insect biomass extracts showed repellent action against T. castaneum, he most active extract being tFBHIMe, with IR values of 26%. Our results indicate that the extracts of Fusarium sp. 3300 are promising for the development of control agents, insect pests and as a potential source of bioactive substances.