Bioactive peptides of the Cry1Ab16 toxin from Bacillus thuringiensis by nanodevices films for potential biotechnological applications

PLACIDO ALEXANDRA; OLIVEIRA FARIAS EMANUEL A.; MARANI MARIELA M.; VASCONCELOS ADREANNE G.; MAFUD ANA C.; MASCARENHAS YVONNE P.; EIRAS CARLA; LEITE JOSÉ R. S. A.; DELERUE-MATOS MARÍA C.

Porto

Encuentro; 15th Iberian Peptide Meeting; 2016

Faculdade de Ciencias-Universidade do Porto

Peptides are potential candidates to meet theneeds of the modern world in relation to diagnosis, disease monitoring, qualitycontrol in industry, and more recently, detection of genetically modifiedorganisms (GMOs) and food security through the development of biosensors(Plácido et al., 2015). Cry1Ab16 is atoxin of crystalline insecticidal proteins family that has been widely used in GMOsto gain resistance to pests. Recently, many studies have focused on evaluatingthe potential environmental impact of this toxin, including the impact onaquatic environments. Moreover, this toxin has been detected in maternal andfetal samples during pesticide exposure studies related to genetically modifiedfood. For the first time, in this study, peptides derived from the immunogenicCry1Ab16 toxin (from Bacillusthuringiensis) were immobilized as layer-by-layer films (Farias et al., 2015). Given the concern aboutfood and environmental safety, a peptide with immunogenic potential,PcL342-354C, was selected for characterization of the electrochemical. Inaddition, an attempt was made to optimize the electrochemical signal of thepeptide through interspersion with different natural or synthetic polymers. Finally,the system was characterized using various morphological and spectroscopictechniques such as AFM, UV-Vis, XRD, and FTIR. An ITO/PEI/(PSS/PcL342-354C)nfilm proved to be an excellent candidate for applications in electrochemicalsensors and other biotechnological applications for GMOs and environmentalindicators.