Formulation of adapted diets for in vivo studies of chronic noncommunicable diseases.

COLUSSI NARELLA ANTONINA; MELANA COLAVITA JUAN PABLO; RODRÍGUEZ JUAN PABLO; TODARO JUAN SANTIAGO; CHAVES MARÍA GUADALUPE; ROMERO VIDOMLANSKY PATRICIA RUTH; ACEVEDO BELÉN; AGUIRRE MARÍA VICTORIA

Buenos Aires

Jornada; X Jornadas anuales de la Sociedad Argentina de Biología; 2018

Sociedad Argentina de Biología

The laboratory mouse is a preeminent study tool in modern experimental research, natural food ingredients such as chia seeds (Salvia hispanica), rich in essential fatty acids represent an innovative alternative for the production of food destined to murine. The objective was to formulate foods rich in essential fatty acids from crushed chia seeds and various protein sources for the establishment of experimental models applicable in studies of chronic non-communicable diseases. Male mice of the Balb-c strain (2 months old) of the Faculty of Medicine Bioterium were randomly distributed in four lots (4 animals each) and provided with water and food ad libitum. Lots were identified as Control group 1= diet A (commercial balanced X); Control group 2= diet B (commercial balanced Y); Experimental group 1= diet C (crushed chia seeds, fish thorn and crushed cowpea (Vigna unguiculata), experimental group 2= diet D (crushed chia seeds and skimmed milk powder). Diets C and D were designed according to the nutritional requirements of Balb-c mice. Pellets were made handcrafted according to dry heat sterilization (80°C-120´)/microwave (100g-1´-1425 MHz)/UV irradiation (254 nm-15´) seeking the optimal conservation and maintanance of good texture. The animals were fed for 1 month and were monitored weekly by weighing and direct observation. Consumed food was also measured. The average weight of mice from lots C and D was 28.6±1.9g and 28.4±1.9g. Weekly average of food consumption was 28.2±5.2g for diet C and 23.5±5.2g for diet D, framed in normality. Biochemical determinations were assessed from blood samples obtained by cardiac puncture under anesthesia. Mice fed with diet C showed: cholesterolemia 57.7±2.86mg/dl (RV 63-174mg/dl), glycemia 113.7±30mg/dl (RV 106-278mg/dl) and triglyceridemia 76.2±17.7mg/dl (RV 71-164mg/dl). Finally, this study revealed that the diet C formulation was accepted preferably by mice and confers them favorable health conditions. Additionally, the most efficient technology for pellets preservation was UV irradiation.