INSTITUTO DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Production of enzyme cocktails by fungal consortiums
PELEGRINI MALPIEDI, LUCIANA; WOITOVICH VALETTI, NADIA; MORILLA, ESTEBAN AMADOR; TUBIO, GISELA
Simposio; Biocatalysis Open Day 2020; 2020
European Federation of Biotechnology
The production of enzymes that constitute enzyme cocktails is carried out by fungal fermentations consisting of an association of different strains coexisting in a single fermentation step. These mixed fermentations are industrially and economically potential because the costs and production times of enzyme cocktails can be considerably reduced.The aim of this work was to evaluate the feasibility of using a fungal consortium by Aspergillus niger and oryzae, and Trichoderma harzanium for the production of an enzyme cocktail enriched in xylanolytic (XE), cellulolytic (CE), amylolytic (AE), proteolytic (PE) and lipidic (LE) enzymes using agro-industrial waste like wheat bran, soybean husk and sunflower husk as substrates. Then, the strains were evaluated to coexist in the same culture by interaction studies and the production of antagonistic metabolites. Different fungal consortium models were tested in selected agro-industrial waste.Solid-state (SSF) and submerged (SmF) fermentation were assayed for 5 days at 30°C in all agro-industrial wastes. The activity of XE, CE and AE were measured by the dinitrosalicylic acid colorimetric method, PE activity was measured by the azocasein method and LE by the colorimetric method based on the hydrolysis of pNPB.The combination of wheat bran and soybean husk proved to be the most favorable source of C and N for maximum enzyme production after 4 days of fermentation at 30°C for A.niger and oryzae. These species demonstrated to be able to coexist in a fungal consortium because no mycelium could occupy the territory occupied by the other microorganism. A. niger and A. oryzae formed a fungal consortium that produced IU/gds of substrate of XE (65), CE (30), AE (135), PE (13,000) and LE (1.95).The results obtained suggest that the fungal consortia consisting of A. niger and A. oryzae was an efficient design for the production of hydrolytic enzyme cocktails.