INSTITUTO DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Application of aqueous micellar systems to purify polyphenolic compounds from chia seeds
VALERIA BOERIS; MARÍA CELESTE GAUDENZIO; LUCIANA PELLEGRINI MALPIEDI
Congreso; BPP2019 - Biopartitioning & Purification Conference; 2019
In the last years, chia seeds have been considered as a functional food whose daily consumption could contribute to improve human health. Among their recognized benefits, it can be mentioned their high antioxidant activity, which is associated with the presence of polyphenolic compounds (PC) and tocopherols. Polyphenols are structurally characterized by the presence of one or more phenol units. In recent years, PC have received a considerable amount of attention as potential natural antioxidants. The most commonly used methodologies to recover PC of biological nature are based upon organic solvent extractions followed by fractionation and clean up. However, these techniques are both expensive and not environmentally friendly due to the high organic solvents consumption. In this context, the aim of this work was to evaluate the extraction efficiency of total PC from chia seeds by the application of aqueous micellar systems (AMTPS) instead of conventional solvents. These assays were accomplished by using Genapol X-080 and Tergitol 15-S-7 (5% w/w), dissolved in distillated water or sodium citrate 100 mM solutions, with a regulated pH of 5.00 or 7.00. Triturated chia seeds were applied without previous treatment, in a solvent to solid ratio of 20. For comparative purposes, the same procedure was accomplished by using acetone, ethanol, methanol and etilacetate as extractive solvents. In all cases, the work temperature was 22°C and the experiments were repeated at least for duplicate. The extractive efficiency was evaluated by PC concentration and antioxidant activity (AA) determinations by using Folin?Ciocalteu (FCA) and DPPH assay, respectively. In all cases, chia seeds remained in the bottom of the test tubes, while the PC were partitioned to the top micelle-rich phase. Among the different extractive conditions, the AMTPS containing Genapol X-080, pH 7.00, possessed the highest extraction efficiency (9.30 mg of galic acid equivalent per gram of dried chia seeds) in comparison to other surfactant formulations and organic solvents. However, the highest AA was observed for the AMTPS containing Tergitol 15-S-7, pH 7.00 (21 mg of trolox equivalent per gram of dried chia seeds). This behavior can be attributed to different types of PC obtained for each surfactant. Neverthelles, further studies should be done in order to explain these results. Considering all the obtained results, it can be concluded that studied AMTPS could be used as a greener alternative to extract PC from chia seeds.