EXTRACTION AND PURIFICATION OF PEROXIDASE AND A TRIPSIN INHIBITOR FROM SOYBEAN HULLS: A STRATEGY TO REVALUE A WASTE AS A SOURCE OF DIFFERENT TYPES OF MOLECULES OF BIOTECHNOLOGICAL INTEREST

GIORDANO, E.D.; WOITOVICH VALETTI, N.; SILVA, J.M.; CAMISCIA, P.; PICÓ, G.A.

Guarajá

Congreso; 20th Biopartitioning & Purification Conference 2019 (BPP 2019); 2019

In our country, one of the most abundant crops is soybean (Glicine max). During their processing, soybean hulls (SBH) are released which corresponds to 2% of its total mass. A small portion of this is used as cattle supplements, but most of it is burned or discarded leading to contamination. SBH contains about 11% protein, 9-11% galactomannans, 10-12% acidic polysaccharides, 9-10% hemicelluloses, 40% cellulose and 1% lignin. The main objective of this work is to extract proteins present in the SBH by lixiviation and the purification of Peroxidase (POD) and a Trypsin inhibitor (TI) by adsorption.To obtain the best extraction performance a general full factorial design was used to screen which were the factors that significantly influenced the extraction process. The following independent variables were tested: SBH mass/aqueous phase volume ratio, contact time between the phases, agitation type and particle size of SBH. As dependent variables were assayed, enzymatic activity of POD and TI since both have great industrial applications. The statistical analysis showed that the factors: contact time, mass/volume ratio and particle size affects significantly the extraction process (p-value