INSTITUTO DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
The use of thermosensitive micellar systems as selective platforms of extraction
FILIPA A. VICENTE; ADALBERTO PESSOA JR; FRANCISCA A. SILVA; SÓNIA P.M. VENTURA; LUCIANA PELLEGRINI MALPIEDI; JOÃO A.P. COUTINHO
Simposio; European Symposium on Biochemical Engineering Sciences (ESBES) 2016; 2016
Aqueous micellar two-phase systems (AMTPS) using surfactants are advantageous thermosensititve extraction systems, since they are mostly composed of water, emerging as an appellative type of liquid-liquid extraction (LLE). AMTPS composed of two surfactants enhanced the extraction of biomolecules, hence exploiting the advantage of introducing ionic liquids (ILs) as a new class of co-surfactants came as an attractive alternative due to their unique properties. Thus, the aim of this work was the development of new micellar systems with potential to be applied into the extraction field. Those were firstly studied considering the design of AMTPS, based on Triton X-114 as the main surfactant by testing two distinct families of ILs, corresponding to the imidazolium and phosphonium. In order to evaluate the extractive potential of these new AMTPS, the partition of different molecules was investigated, considering the effect of the ILs as co-surfactants in the coexistence curves and their impact upon the solutes partition. For both molecules under study, cytochrome c (Cyt c) and Rhodamine 6G (R6G), the partition coefficients (K) and selectivities (SR6G/Cyt c) were assessed. The obtained results clearly demonstrate that ILs have a major effect on the Tcloud. The presence of ILs as co-surfactants was able, not only to enhance the KCyt c (indicated as log KCyt c) from -0.587 ± 0.117 up to -1.510 ± 0.139, but also to improve the SR6G/Cyt c from 925.25 up to 3418.89.