IPROBYQ   25157
INSTITUTO DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Bromelain isolation from pineapple residues by carrageenan complex formation
Autor/es:
LORENZO M. PASTRANA; GUILLERMO PICÓ; NADIA WOITOVICH VALETTI; MARIA M PINTADO; DÉBORA A. CAMPOS; JOSÉ A. TEIXEIRA
Lugar:
Salamanca
Reunión:
Congreso; Congreso Iberoamericano de Biotecnología- BioIberoamérica 2016; 2016
Resumen:
The isolation and purification of bioproducts are very important processes in the biotechnology industry, representing 80?90% of total production costs. Furthermore, the development of simple and viable methods for protein purification has been an essential pre-requisite for many advances in biotechnology (Harikrishna et al.,2002).Bromelain (BR) is a crude, aqueous extract from the stems and immature fruits of pineapples (Ananas comosus) and can be found in the tissue of plants of the Bromeliaceae family and belongs to cysteine-proteinases family (Rowan et al., 1990). Several processes of extraction were developed, but all with some disadvantage (high cost, low purity, high use of chemical products). From an industrial view (low cost of production) the most suitable was the simplest method; a cooled pineapple juice centrifuged, ultrafiltrated and lyophilized, constituting an unusually complex mixture of different type of compounds, interfering with BR bioavailability. Two types of BR were describe, from stem (EC. 3.4.22.32) and from fruit (EC. 3.4.22.33), previously called bromelin (Cooreman et al., 1978).These group of enzymes offer a wide spectrum of therapeutic efficacies: they demonstrate, in vitro and in vivo, antiedemateous, antiinflammatory, antithrombotic and fibrinolytic activities (Maurer et al., 2001), increasing the importance of determining a viable extraction and purification method for this enzyme. A new method of purification and isolation of Bromelain was developed, using a natural food safe polysaccharide (carrageenan).Carrageenan (Carr) is derived from certain species of red seaweeds (Rhodophyceae) and shows a wide range of rheological and functional properties. Previous reports have demonstrated the use of this polysaccharide to isolate and immobilize enzymes (Fabian et al., 2010).A complex formation of BR-Carr was studied and improved to obtain an isolate BR by precipitation. The BR-Carr was evaluated by several techniques, such as DLS (dynamic light scattering), FPLC (high-resolution fast protein liquid chromatography) and biochemical methods (Bromelain activity).Based on this, it was possible to separate and precipitate BR from an aqueous extract maintaining its activity. Although it was possible to obtain a first step in the purification, in the future other successive steps will be evaluated to increase the purity.