INSTITUTO DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Selective Micellar Extraction using Ionic Liquids as Co-Surfactants
FILIPA A. VICENTE; LUCIANA PELLEGRINI MALPIEDI; FRANCISCA A. E SILVA; ADALBERTO PESSOA JR; JOÃO A.P. COUTINHO; SÓNIA P.M. VENTURA
Conferencia; Biopartitioning & Purification Conference 2015; 2015
Aqueous micellar two-phase systems (AMTPS) using surfactants have been applied as advantageous extraction systems, since they are mostly composed of water, emerging as a further appellative type of liquid-liquid extraction (LLE). A plethora of biomolecules have already been separated and purified through these systems with several surfactants. AMTPS composed by two surfactants enhanced the extraction of biomolecules, hence exploiting the advantage of a co-surfactant by using ionic liquids (ILs) as a new class of surfactants came as an attractive alternative due to their unique properties. Thus, the objective was to develop a new technology to be applied into the extraction field.In this work, two families of ILs, namely the [Cnmim] and phosphonium were applied in the design of new AMTPS, based in Triton X-114 as the main surfactant. To evaluate the potential of this new LLE system, the partition and purification of different (bio)molecules is here analyzed considering the effect of the ILs as co-surfactants in the coexistence curves and their impact upon the solutes partition. Therefore, two (bio)molecules, cytochrome c (Cyt c) and Rhodamine 6G, are used in order to study their partition coefficient (K) and their selectivity (S) in the mentioned AMTPS. The obtained results clearly demonstrate that ILs have an important effect on the Tcloud. The presence of ILs as co-surfactants is able not only to enhance the KCyt c (indicated as log KCyt c) from -0.587 ± 0.117 up to -1.510 ± 0.139, but also to improve the selectivity parameter (SR6G/Cyt c) from 925.25 up to 3418.89.