INSTITUTO DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
An industrial scale process for the enzymatic removal of steryl glucosides from biodiesel
PEIRU, S.; AGUIRRE, A; EBERHARDT, F.; BRAIA, M.; CABRERA, R.; MENZELLA, H.
Congreso; THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES 2015; 2015
Biodiesels produced from lipid transesterification of vegetable oils have a major quality problem due to the presence of precipitates, which need to be removed to avoid clogging of filters and engine failures.These precipitates have been reported to be mostly composed of steryl glucosides (SGs), but so far industrial cost-effective methods to remove these compounds are not available. Here we describe a novel method for the efficient removal of SGs from biodiesel, based on the hydrolytic activity of a thermostable β-glycosidase obtained from Thermococcus litoralis. This steryl glucosidase (SGase)enzyme was efficiently produced and purified from Escherichia coli cultures expressing a syntheticcodon-optimized gene, and used to treat soybean derived biodiesel. Several optimization steps allowed for the selection of optimal reaction conditions to finally provide a simple and efficient process for the removal of SGs from crude biodiesel. The resulting biodiesel displayed excellent filterability properties according to the Total Contamination (TC), the Cold Soak Filtration Test (CSFT), Filter Blocking Tendency (FBT), and Cold Soak Filter Blocking Tendency (CSFBT) tests. The process was successfully scaled up to a 20 ton reactor, confirming its adaptability to industrial settings without significant capital investments. The results presented in this work provide a novel path for the removal of steryl glucosides from biodiesel using a cost-effective, environmentally friendly and scalable enzymatic process, contributing to the adoption of this renewable fuel.