IPROBYQ   25157
INSTITUTO DE PROCESOS BIOTECNOLOGICOS Y QUIMICOS ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Under the Development of Aqueous Micellar Extraction Systems using Ionic Liquids as Co-surfactants
Autor/es:
FILIPA A. VICENTE; LUCIANA PELLEGRINI MALPIEDI; FRANCISCA A. SILVA; ADALBERTO PESSOA JR; JOÃO A.P. COUTINHO; SÓNIA P.M. VENTURA
Lugar:
Madrid
Reunión:
Congreso; Iberoamerican Meeting on Ionic Liquids; 2015
Institución organizadora:
Universidad Autónoma de Madrid
Resumen:
Aqueous micellar two-phase systems (AMTPS) using surfactants are advantageous extraction systems due to their main aqueous component, emerging as an appellative type of liquid-liquid extraction (LLE). A plethora of biomolecules have already been separated and purified using these systems with several surfactants. AMTPS composed of two surfactants enhanced the extraction of biomolecules, hence exploiting the advantage of introducing ionic liquids (ILs) as a new class of co-surfactants came as an attractive alternative owing to their unique properties. The alkylmethylimidazolium [Cnmim] family has been widely studied and demonstrated to self-aggregate in micelles, having also reports on their incorporation in mixed micelles. Thus, the objective of this work is to develop a new technology to be applied into the extraction field.Herein, two families of ILs, namely the [Cnmim] and phosphonium were applied in the design of new AMTPS, based on Triton X-114 as the main surfactant. To evaluate the potential of this new LLE system, the partition and purification of different (bio)molecules is analyzed, considering the effect of the ILs as co-surfactants in the coexistence curves and their impact upon the solutes partition. Therefore, two (bio)molecules, cytochrome c (Cyt c) and Rhodamine 6G (R6G), are used in order to study their partition coefficients (K) and selectivities (SR6G/Cyt c)in the mentioned AMTPS. The obtained results clearly demonstrate that ILs have an important effect on the Tcloud. The presence of ILs as co-surfactants is able not only to enhance the KCyt c (indicated as log KCyt c) from -0.587 ± 0.117 up to -1.510 ± 0.139, but also to improve the SR6G/Cyt c from 925.25 up to 3418.89