Peptides from Amaranth controlled the NF-κB pathway activation on epithelial cells and suppressed intestinal inflammation

RIZZO, GASTÓN; AÑON, CRISTINA; QUEREDA, MICAELA; MORONTA, JULIAN; SMALDINI, PAOLA; ORSINI DELGADO, MARIA LUCIA; DOCENA, GUILLERMO

Mar del Plata

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología; 2018

Biological, nutritional and health benefits of amaranth have been highlighted in the last years.Proteins of amaranth exert anti-hypertensive, anti-oxidant, anti-thrombotic and anti-proliferative effects.The aim of this study was to analyze the anti-inflammatory effect of peptides from amaranth on NF-kB-intracellular pathway activation in intestinal epithelial cells, and in experimental intestinal inflammation,such as colitis and food allergy.Colon cell lines (Caco-2 and Caco-luc) were cultured with flagellin and amaranth peptides. CCL20-expression was evaluated by qPCR and NF-κB modulation was evaluation by light emission and qPCR,along with p65-nuclear traslocation. In vivo studies included the oral administration of a formulationcontaining the peptide during the allergic sensitization or the colitis induction phase in Balb/c mice.Treatment efficacy was in vivo and in vitro evaluated.We found several peptides with anti-inflammatory capacity and we selected that with the highestability to suppress cell activation (decrease in CCL20 and light emission p