athogen-specific T cells from Patients with Inflammatory Bowel Diseases can be Modulated by Probiotics from Kefir.

CANZIANI, K.; SERRADELL, M; HUGO, A; BRAYER, S; CORREA G; VILLAVERDE, A; CURCIARELLO, R; BARBIERA, E; RUMBO, M; ROCCA, A; YANTORNO, M; TUFARÉ F; MUGLIA C.I.; SALTO, I; ERREA, A; ALVAREZ, MC; SAMBUELLI, A.; GARBI, L; DOCENA, G.

Mar del Plata

Congreso; SAI-SAIC-SAFIS 2018; 2018

Kefir is a fermented milk with health-promoting properties. Lamina propria T cells (LPTC) are central in inflammatory Bowel Disease (IBD) pathogenesis, contributing to mucosal inflammation by secreting pro-inflammatory cytokines and being resistant to apoptosis. We aimed to modulate proliferation and the pro-inflamatory response of pathogen specific LPTC from IBD patients with microorganisms from kefir.Colonic biopsies from IBD patients were incubated with probiotics from kefir and cytokines in supernatants were evaluated by ELISA. Alternatively, biopsies (N=23; 7 CD and 15 CU) were washed in HBSS medium with EDTA and DTT, and digested by collagenase and DNAse treatment. In order to enrich pathogen-specific T lymphocytes, cells were cultured with extracts of enteroadhesive (EA) Escherichia coli and IL-2 for 10 days. Microorganisms from kefir (Lactobacillus kefiri and Enterococcus durans), their conditioned media or 10 µM lactate (abundant in culture supernantants) were evaluated for their modulation capacity on LPTC by proliferation assays (CFSE) and cytokine secretion (TNF-α, IFN-γ, IL-6, IL-10 and IL-13 by ELISA) of LPTC stimulated with anti-CD3/CD28.Supernatants of biopsies incubated with probiotics presented lower levels, compared to controls, of pro-inflammatory cytokines IL-6 (1800±670 and 576±3 vs 6900±2000 pg/ml respectively) and IL-8 (38000±11000 and 32000±7300 vs 80000±27000 pg/ml respectively). LPTC lines specific for EA E. coli were developed for all patients. Cell proliferation of activated CD4+/CFSE-/PI- LPTC decreased significantly with L. kefiri and E. durans and lactate (P