Role of Galectin 1 in the pathogenesis of Inflammatory Bowel Diseases

PAPA GOBBI, R; ROCCA, A; SAMBUELLI, A.; CURCIARELLO, R; YANTORNO, M; CORREA G; MOROSI L; MARIÑO K; TOSCANO, M.; MUGLIA C.I.; RABINOVICH, G.; DOCENA, G.

Buenos Aires

Congreso; IV LASID Meeting LXIII Argentinean Immunology Society Meeting II French-Argentinean Immunology Meeting; 2015

Abstract: Background: Inflammatory bowel diseases (IBD) are chronic and relapsing intestinal inflammatory disorders. Galectin-1 (Gal-1) is a lectin with anti-inflammatory properties and therapeutic potential in immune-mediated disorders. In this study we investigated the role of Gal-1 in the colonic mucosa of patients with IBD.Methods: Intestinal specimens from control (HC) (n=46) and IBD patients (n=82) were studied by RT-qPCR, immunoblotting, immunohistochemistry and ELISA for Gal-1 production and flow cytometry for detection of Gal-1-specific ligands in lamina propria T cells (LPTC). Gal-1 functional assays were performed by flow cytometry (Annexin V) and TUNEL assay.RESULTS: We found Gal-1 mRNA expression in colonic inflamed areas (p