Study of acute cellular rejection process in an experimental model of small bowel transplantation

STRINGA, PABLO; LAUSADA, NATALIA; ROMANIN, DAVID; PORTIANSKY, ENRIQUE; MARTÍN, PEDRO; ABATE, JUAN CRUZ; VECCHIO, LEANDRO; ZANUZZI, CAROLINA; MILESI, MARIA VERÓNICA; GONDOLESI, GABRIEL; RUMBO, MARTIN

Buenos Aires

Congreso; IV Latin American Society for Immunodeficiencies (LASID), LXIII Reunión Anual de la Sociedad Argentina de Inmunología (SAI), II French-Argentinean Immunology Meeting; 2015

LASID - SAI - FAIC

Abstract: Graft rejection (GR) is a major clinical complication in intestinal transplant. Experimental models are essential to better understand the immunobiology of GR. Our aim is to evaluate GR process in an heterotopic intestinal transplantation (HIT) model in rats.Five syngeneic and 5 allogeneic HIT were performed. Graft and blood were sampled at 5-7 and 10-12 days after HIT. Histopathological studies, quantification of apoptotic cells and analysis of IL-6 expresion by RTQ-PCR on the entire graft and in different compartments (epithelial, muscular layer and serosa) isolated by microscopy disection were performed. Nitrite plasma levels were determined as a marker of GR.Allogeneic group showed Mild (3/5) and moderate rejection (2/5) at 5-7 pos-HIT day. Severe rejection were observed in all allogeneic samples at 10-12 pos-HIT days. Isogenic group showed no histological changes. Apoptotic cells increased in allogeneic grafts (512 and 2021 x10 fields at 5 and 10-12 post-HIT days respectively). IL-6 increased by 100 times in allogeneic graft (p