Peptides of Amaranth were targeted as containing sequences with potential anti-inflammatory properties

MORONTA, JULIAN; AÑON, MARIA CRISTINA; SMALDINI PAOLA LORENA; DOCENA GUILLERMO H

Journal of Functional Foods

Elsevier

Año: 2016 vol. 21 p. 463 - 473

1756-4646

The immunomodulatory effect of amaranth peptides on epithelial cellsactivated through the NF-κB signallingpathway was examined. Results showed that extensive protein hydrolysis from amaranth(23and30% degree of hydrolysis) reduced the emission of light inbacterial flagellin -activated Caco-2 CCL20: luc cells (Caco-2 cellstransfected with a luciferase reporter under the control of the CCL20 promoter)compared with the non-hydrolyzed protein. Purification of themost active peptide fractions by HPLC chromatography and sequencing showed thatthe peptide SSEDIKE possessed a modulatory capacity onactivated cells to suppress the expression of mRNA coding for CCL20. Thispeptide was non-toxic for cells. These findings indicated that thepeptide SSEDIKE derived from proteins of amaranth attenuated the activation ofhuman intestinal epithelial cell, and hence, Amaranth proteins could be included in functional foods as asource of bioactive peptides with health promoting properties.