Orally-induced intestinal CD4+ CD25+FoxP3+ Treg Controlled Undesired Responses towards Oral Antigens and Effectively Dampened Food Allergic Reactions

SMALDINI PAOLA LORENA; ORSINI DELGADO, M LUCÍA; FOSSATI, CARLOS A; DOCENA, GUILLERMO H

PLOS ONE

PUBLIC LIBRARY SCIENCE

Lugar: San Francisco; Año: 2015

1932-6203

The induction of peripheral tolerancemay constitute a disease-modifying treatment for allergic patients. We studiedhow oral immunotherapy (OIT) with milk proteins controlled allergy insensitized mice (cholera toxin plus milk proteins) upon exposure to theallergen. Symptoms were alleviated, skin test was negativized, serum specificIgE and IgG1 were abrogated, a substantial reduction in the secretion of IL-5and IL-13 by antigen-stimulated spleen cells was observed, while IL-13 geneexpression in jejunum was down-regulated, and IL-10 and TGF-β were increased. Inaddition, we observed an induction of CD4+CD25+FoxP3+cells and IL-10- and TGF-β-producing regulatory T cells in the lamina propria.Finally, transfer experiments confirmed the central role of these cells intolerance induction. We demonstrated that the oral administration of milkproteins pre- or post-sensitization controlled the Th2-immune response throughthe elicitation of mucosal IL-10- and TGF-β-producing Tregs that inhibitedhypersensitivity symptoms and the allergic response.