APPLICATION OF Z-SINAPINIC MATRIX IN PEPTIDE MALDI-MS ANALYSIS

SALUM, M.L.; CAMPERI, S.A.; GIUDICESSI, S.L.; ERRA-BALSELLS, R.; SCHMIDT DE LEÓN, T.

JOURNAL OF MASS SPECTROMETRY

JOHN WILEY & SONS LTD

Lugar: LOndres; Año: 2017 vol. 52 p. 182 - 186

1076-5174

Since introduction of sinapinic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices successful application of matrix assisted laser desorption/ionization mass spectrometry started for protein/polypeptides. Both show some limitations in short peptide analysis because matrix clusters are quite abundant. Cinnamics currently used are E-cinnamics. Here Z-SA as matrix for peptides is studied and compared with E-SA and CHCA. Minor number of clusters are always observed in the low m/z region allowing the detection of short peptides. The results here described show that this novel matrix is a tool of choice for direct, rapid and sensitive detection of hydrophilic and hydrophobic peptides.